Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Fibroblast growth factor-inducible 14 accelerates pulmonary fibrosis by inducing fibroblast senescence in mice

doi: 10.1007/s00018-026-06161-w

Figure Lengend Snippet: Activation of Fn14 activates the cGAS‑STING pathway in fibroblasts. A , primary fibroblasts were treated with rTWEAK (100 ng/mL) for 48 h. Immunofluorescence and confocal microscopy show the colocalization of Picogreen (green) and Mitotracker (red) in rTWEAK-treated primary fibroblasts, scale bar = 50 μm. B , D-G , The protein levels of cGAS, STING, p-TBK1 Ser172 , and p-IRF3 Ser396 were determined by Western blot ( n = 3). C , Immunofluorescence and confocal microscopy show the colocalization of STING (green) and GM130 (red) in rTWEAK-treated primary fibroblasts, scale bar = 50 μm. * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: To evaluate the role of the cGAS-STING signaling in rTWEAK-induced primary fibroblast senescence, we treated cells with the cGAS inhibitor (RU.521, 10 μM, MedChemExpress, USA) 30 min before the rTWEAK stimulation.

Techniques: Activation Assay, Immunofluorescence, Confocal Microscopy, Western Blot

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Fibroblast growth factor-inducible 14 accelerates pulmonary fibrosis by inducing fibroblast senescence in mice

doi: 10.1007/s00018-026-06161-w

Figure Lengend Snippet: Inhibition of the cGAS-STING signaling suppresses the senescence of rTWEAK-treated fibroblasts. A-B , primary fibroblasts were treated with rTWEAK (100 ng/mL) for 48 h after RU.521 (10 µM) intervention for 30 min. Western blot was conducted to examine the expression levels of cGAS, STING, p-TBK1 Ser172 , and p-IRF3 Ser396 ( n = 3). C-D , The senescence-related protein levels of P53, P21, P16, and γ-H2AX were assessed by Western blot ( n = 3). E , Immunofluorescence of Ki67 protein expression in rTWEAK-treated primary fibroblasts with RU.521, scale bar = 50 μm. F , Comet assay in rTWEAK-treated primary fibroblasts with RU.521, scale bar = 10 μm. G , Real-time PCR for Il-6 , Tgf-β , Mcp1 , Mmp2 , Mmp9 , and Mmp12 mRNA expression ( n = 3). H , SA-β-gal staining was performed 48 h after rTWEAK treatment, scale bar = 50 μm. * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: To evaluate the role of the cGAS-STING signaling in rTWEAK-induced primary fibroblast senescence, we treated cells with the cGAS inhibitor (RU.521, 10 μM, MedChemExpress, USA) 30 min before the rTWEAK stimulation.

Techniques: Inhibition, Western Blot, Expressing, Immunofluorescence, Single Cell Gel Electrophoresis, Real-time Polymerase Chain Reaction, Staining

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Fibroblast growth factor-inducible 14 accelerates pulmonary fibrosis by inducing fibroblast senescence in mice

doi: 10.1007/s00018-026-06161-w

Figure Lengend Snippet: Schematic illustration. Fn14 is up-regulated in fibroblasts of PF and serves as a pro-senescent factor. Fn14 triggers fibroblast senescence by impairing mitophagy, resulting in mtDNA release and activation of cGAS-STING signaling

Article Snippet: To evaluate the role of the cGAS-STING signaling in rTWEAK-induced primary fibroblast senescence, we treated cells with the cGAS inhibitor (RU.521, 10 μM, MedChemExpress, USA) 30 min before the rTWEAK stimulation.

Techniques: Activation Assay